Papers

600 records — page 2 of 3

• Improved vectors and genome-wide libraries for CRISPR screening
• AJCC Cancer Staging Manual
• Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
• [19] Rapid and efficient site-specific mutagenesis without phenotypic selection
• High-efficiency transformation of mammalian cells by plasmid DNA.
• PD-L2 is a second ligand for PD-1 and inhibits T cell activation
• CTLA-4 Control over Foxp3 ⁺ Regulatory T Cell Function
• The history and advances in cancer immunotherapy: understanding the characteristics of tumor-infiltrating immune cells and their therapeutic implications
• Melanoma-intrinsic β-catenin signalling prevents anti-tumour immunity
• Delivery technologies for cancer immunotherapy
• Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression
• A Simple and General Method for Transferring Genes into Plants
• Analysis of 100,000 human cancer genomes reveals the landscape of tumor mutational burden
• The evolving tumor microenvironment: From cancer initiation to metastatic outgrowth
• Intratumoral T Cells, Recurrence, and Survival in Epithelial Ovarian Cancer
• Exosomal PD-L1 contributes to immunosuppression and is associated with anti-PD-1 response
• Inflammation and tumor progression: signaling pathways and targeted intervention
• A view on drug resistance in cancer
• The evaluation of tumor-infiltrating lymphocytes (TILs) in breast cancer: recommendations by an International TILs Working Group 2014
• Search-and-replace genome editing without double-strand breaks or donor DNA
• KEGG: integrating viruses and cellular organisms
• Full-length RNA-seq from single cells using Smart-seq2
• Core Transcriptional Regulatory Circuitry in Human Embryonic Stem Cells
• CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity
• Master Transcription Factors and Mediator Establish Super-Enhancers at Key Cell Identity Genes
• Neoadjuvant Nivolumab plus Chemotherapy in Resectable Lung Cancer
• Pembrolizumab in patients with advanced hepatocellular carcinoma previously treated with sorafenib (KEYNOTE-224): a non-randomised, open-label phase 2 trial
• The DNA Damage Response: Making It Safe to Play with Knives
• Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
• Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage
• Generation of germline-competent induced pluripotent stem cells
• Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells
• Tumor Mutational Burden as an Independent Predictor of Response to Immunotherapy in Diverse Cancers
• Systemic Immune-Inflammation Index Predicts Prognosis of Patients after Curative Resection for Hepatocellular Carcinoma
• Immune Checkpoint Targeting in Cancer Therapy: Toward Combination Strategies with Curative Potential
• One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering
• Efficient transformation of rice (<i>Oryza sativa</i> L.) mediated by <i>Agrobacterium</i> and sequence analysis of the boundaries of the T‐DNA
• Designer deletion strains derived fromSaccharomyces cerevisiae S288C: A useful set of strains and plasmids for PCR-mediated gene disruption and other applications
• Specific killing of BRCA2-deficient tumours with inhibitors of poly(ADP-ribose) polymerase
• Genome-Wide Insertional Mutagenesis of <i>Arabidopsis thaliana</i>
• The nuclear RNase III Drosha initiates microRNA processing
• Production of high-titer helper-free retroviruses by transient transfection.
• The ground state of embryonic stem cell self-renewal
• RNA interference
• Class Switch Recombination and Hypermutation Require Activation-Induced Cytidine Deaminase (AID), a Potential RNA Editing Enzyme
• Expression of a single transfected cDNA converts fibroblasts to myoblasts
• Formation of Pluripotent Stem Cells in the Mammalian Embryo Depends on the POU Transcription Factor Oct4
• A Third-Generation Lentivirus Vector with a Conditional Packaging System
• Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity
• PD-L1 Expression as a Predictive Biomarker in Cancer Immunotherapy
• CTLA-4 and PD-1 Pathways
• Crosstalk between cancer-associated fibroblasts and immune cells in the tumor microenvironment: new findings and future perspectives
• Chimeric Antigen Receptor Therapy
• The PD‐1 pathway in tolerance and autoimmunity
• THE B7 FAMILY REVISITED
• PD-1 expression by tumour-associated macrophages inhibits phagocytosis and tumour immunity
• High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells
• Regulation of Translation Initiation in Eukaryotes: Mechanisms and Biological Targets
• RNA interference is mediated by 21- and 22-nucleotide RNAs
• The druggable genome
• Improved method for high efficiency transformation of intact yeast cells
• Immune Checkpoint Inhibitors for the Treatment of Cancer: Clinical Impact and Mechanisms of Response and Resistance
• Immunologic Self-Tolerance Maintained by Cd25+Cd4+Regulatory T Cells Constitutively Expressing Cytotoxic T Lymphocyte–Associated Antigen 4
• Cytotoxic T Lymphocyte–Associated Antigen 4 Plays an Essential Role in the Function of Cd25+Cd4+ Regulatory Cells That Control Intestinal Inflammation
• Roles of the immune system in cancer: from tumor initiation to metastatic progression
• Deep learning in neural networks: An overview
• Functional Expression Cloning of Nanog, a Pluripotency Sustaining Factor in Embryonic Stem Cells
• Efficient gene transfer in C.elegans: extrachromosomal maintenance and integration of transforming sequences.
• Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization.
• The Homeoprotein Nanog Is Required for Maintenance of Pluripotency in Mouse Epiblast and ES Cells
• CRISPR-Cas systems for editing, regulating and targeting genomes
• Molecular Signature of CD8+ T Cell Exhaustion during Chronic Viral Infection
• Tumour-infiltrating lymphocytes and prognosis in different subtypes of breast cancer: a pooled analysis of 3771 patients treated with neoadjuvant therapy
• Recombinant Genomes Which Express Chloramphenicol Acetyltransferase in Mammalian Cells
• A Species of Small Antisense RNA in Posttranscriptional Gene Silencing in Plants
• Direct conversion of fibroblasts to functional neurons by defined factors
• Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries.
• Genetic Transformation of <i>Drosophila</i> with Transposable Element Vectors
• Efficient genome editing in zebrafish using a CRISPR-Cas system
• Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method
• An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells
• mTOR Interacts with Raptor to Form a Nutrient-Sensitive Complex that Signals to the Cell Growth Machinery
• A Common Mechanism of Cellular Death Induced by Bactericidal Antibiotics
• High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method
• New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites
• ClinVar: public archive of interpretations of clinically relevant variants
• Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation
• Reducing the Dimensionality of Data with Neural Networks
• Reprogramming of human somatic cells to pluripotency with defined factors
• The Significance of Digital Gene Expression Profiles
• Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum
• Cas9–crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria
• CRISPR–Cas12-based detection of SARS-CoV-2
• The hitch-hiking effect of a favourable gene
• A DNA damage checkpoint response in telomere-initiated senescence
• Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts
• Mammalian Caspases: Structure, Activation, Substrates, and Functions During Apoptosis
• A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila
• Statistical Analysis With Missing Data
• In vivo genome editing using Staphylococcus aureus Cas9
• High-resolution in situ hybridization to whole-mount zebrafish embryos
• Nonchromosomal Antibiotic Resistance in Bacteria: Genetic Transformation of <i>Escherichia coli</i> by R-Factor DNA
• Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase.
• High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects
• Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro
• The relation of recombination to mutational advance
• Improved Survival with Ipilimumab in Patients with Metastatic Melanoma
• Creation of a Bacterial Cell Controlled by a Chemically Synthesized Genome
• In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state
• Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6
• A Cellular Function for the RNA-Interference Enzyme Dicer in the Maturation of the <i>let-7</i> Small Temporal RNA
• RNAi
• Rationally engineered Cas9 nucleases with improved specificity
• Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes
• Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA
• Smart-seq2 for sensitive full-length transcriptome profiling in single cells
• ‘Touchdown’ PCR to circumvent spurious priming during gene amplification
• Discrete Small RNA-Generating Loci as Master Regulators of Transposon Activity in Drosophila
• ‘Green mice’ as a source of ubiquitous green cells
• A Simple, Positive Semi-Definite, Heteroskedasticity and Autocorrelation Consistent Covariance Matrix
• Easy quantitative assessment of genome editing by sequence trace decomposition
• Heterologous modules for efficient and versatile PCR-based gene targeting inSchizosaccharomyces pombe
• An updated evolutionary classification of CRISPR–Cas systems
• Integration of External Signaling Pathways with the Core Transcriptional Network in Embryonic Stem Cells
• New heterologous modules for classical or PCR‐based gene disruptions in <i>Saccharomyces cerevisiae</i>
• CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III
• Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA
• CRISPOR: intuitive guide selection for CRISPR/Cas9 genome editing experiments and screens
• BlobToolKit – Interactive Quality Assessment of Genome Assemblies
• A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformaion of Escherichia coli
• RNA-guided editing of bacterial genomes using CRISPR-Cas systems
• LIBSVM
• Evolution and classification of the CRISPR–Cas systems
• Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and derived variants
• Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases
• Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
• Regularization and Variable Selection Via the Elastic Net
• The Oct4 and Nanog transcription network regulates pluripotency in mouse embryonic stem cells
• Selection of single-stranded DNA molecules that bind and inhibit human thrombin
• A nuclear factor induced by hypoxia via de novo protein synthesis binds to the human erythropoietin gene enhancer at a site required for transcriptional activation.
• mRNA-based therapeutics — developing a new class of drugs
• In Vivo Protein Transduction: Delivery of a Biologically Active Protein into the Mouse
• A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants
• C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector
• Complete cloning of the duchenne muscular dystrophy (DMD) cDNA and preliminary genomic organization of the DMD gene in normal and affected individuals
• Human Induced Pluripotent Stem Cells Free of Vector and Transgene Sequences
• Density Estimation for Statistics and Data Analysis
• A highly conserved repetitive DNA sequence, (TTAGGG)n, present at the telomeres of human chromosomes.
• Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA
• Derivation of completely cell culture-derived mice from early-passage embryonic stem cells.
• The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA
• Small silencing RNAs: an expanding universe
• GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
• High efficiency transformation of Escherichia coli with plasmids
• In Vivo Analysis of Autophagy in Response to Nutrient Starvation Using Transgenic Mice Expressing a Fluorescent Autophagosome Marker
• Epigenetic memory in induced pluripotent stem cells
• Genome editing with engineered zinc finger nucleases
• Telomerase Catalytic Subunit Homologs from Fission Yeast and Human
• CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing
• Selective organ targeting (SORT) nanoparticles for tissue-specific mRNA delivery and CRISPR–Cas gene editing
• Intervening Sequences of Regularly Spaced Prokaryotic Repeats Derive from Foreign Genetic Elements
• Mechanisms of, and Barriers to, Horizontal Gene Transfer between Bacteria
• Tetrazolium dyes as tools in cell biology: New insights into their cellular reduction
• Animal models of human disease: zebrafish swim into view
• Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
• New cell lines from mouse epiblast share defining features with human embryonic stem cells
• Computational correction of copy number effect improves specificity of CRISPR–Cas9 essentiality screens in cancer cells
• Conditional gene targeting in macrophages and granulocytes using LysMcre mice
• High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier
• RNA-programmed genome editing in human cells
• Isolation and characterization
• RNA targeting with CRISPR–Cas13
• Neural networks and physical systems with emergent collective computational abilities.
• Bad, a heterodimeric partner for Bcl-xL and Bcl-2, displaces bax and promotes cell death
• Maintenance of pluripotency in human and mouse embryonic stem cells through activation of Wnt signaling by a pharmacological GSK-3-specific inhibitor
• Random Forests
• Long Short-Term Memory
• Deep learning
• Gradient-based learning applied to document recognition
• Particle swarm optimization
• A Threshold Selection Method from Gray-Level Histograms
• Model Selection and Multimodel Inference: A Practical Information-Theoretic Approach
• Support-vector networks
• The Nature of Statistical Learning Theory
• Dropout: a simple way to prevent neural networks from overfitting
• Support-Vector Networks
• Learning representations by back-propagating errors
• Neural Networks: A Comprehensive Foundation
• Greedy function approximation: A gradient boosting machine.
• Reinforcement Learning: An Introduction
• Statistical Learning Theory
• Batch Normalization: Accelerating Deep Network Training by Reducing Internal Covariate Shift
• Investigating Causal Relations by Econometric Models and Cross-spectral Methods
• Multilayer feedforward networks are universal approximators
• Perceptual symbol systems
• Classification and Regression Trees.
• Pattern Classification
• Fuzzy identification of systems and its applications to modeling and control
• Bagging Predictors
• Pattern Recognition with Fuzzy Objective Function Algorithms